Journal: Translational Oncology

Article Title: Global Conservation of Protein Status between Cell Lines and Xenografts 1

doi: 10.1016/j.tranon.2016.05.005

Figure Lengend Snippet: Comparison of In Vitro and In Vivo Data for Each Model

Article Snippet: SNB19 glioma cell line was given by N. Auger (Institut Curie, Paris, France).

Techniques: Comparison, In Vitro, In Vivo

Journal: Cancer Management and Research

Article Title: The Potential Significance of the EMILIN3 Gene in Augmenting the Aggressiveness of Low-Grade Gliomas is Noteworthy

doi: 10.2147/CMAR.S463694

Figure Lengend Snippet: EMILIN3 gene function experiment in LN229. qRT-PCR transfected LN229 human glioma cells efficiently (A) . The CCK-8 experiment and live/dead staining findings demonstrated that overexpression of the EMILIN3 gene may increase LN229 cell line proliferation and vitality ( B and C ). Overexpression of the EMILIN3 gene enhances the migration, invasiveness, and colony formation of the LN229 cell line (D-F ).

Article Snippet: The LN229 and HS-683 human glioma cell line were purchased from iCell Bioscience Inc, and grown in DMEM media (Gibco, USA) with 10% foetal bovine serum (BI, USA) and 1g/mL penicillin/streptomycin (Hyclone, USA) at 37 degrees Celsius and 5% carbon dioxide.

Techniques: Quantitative RT-PCR, Transfection, CCK-8 Assay, Staining, Over Expression, Migration

Journal: Frontiers in Oncology

Article Title: System analysis based on the migration- and invasion-related gene sets identifies the infiltration-related genes of glioma

doi: 10.3389/fonc.2023.1075716

Figure Lengend Snippet: EMP3 silencing attenuates the migration and invasion of glioma cells. (A) The expression of EMP3 in different cell lines in the CCLE datasets. (B–D) Western blot and qPCR display EMP3 express highly in U118 and A172. (E–G) The growth curve and plate cloning experiment display that EMP3 silencing has little effect on the cell proliferation of U118 and A172. (H, I) EMP3 knockdown inhibits the migration and invasion of glioma cells. ( J–O , 20×) qPCR displays EMP3 silencing attenuates the expression of MMP2 and MMP9 expression (ns, p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: The normal human astroglia (NHA), which were purchased from the Chongqing Golden Magpie Technology Development Co., and U251, LN229, A172, U118, and U87 cells, which were purchased from the China Center for Type Culture Collection (Shanghai, China), were cultured in the DMEM medium supplemented with 10% FBS (HyClone, UT, USA) and 1% penicillin–streptomycin (Beyotime Biotechnology, Jiangsu, China).

Techniques: Migration, Expressing, Western Blot, Cloning, Knockdown

Journal: Frontiers in Oncology

Article Title: System analysis based on the migration- and invasion-related gene sets identifies the infiltration-related genes of glioma

doi: 10.3389/fonc.2023.1075716

Figure Lengend Snippet: EMP3 silencing suppressed EMT marker expression. (A) Western blot analysis of U118 transfected with indicated siRNAs targeting EMP3 (siEMP3#1, siEMP3#2, and siEMP3#3) or siNC. (B) Western blot analysis of A172 transfected with indicated siRNAs targeting EMP3 (siEMP3#1, siEMP3#2, and siEMP3#3) or siNC. (p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).

Article Snippet: The normal human astroglia (NHA), which were purchased from the Chongqing Golden Magpie Technology Development Co., and U251, LN229, A172, U118, and U87 cells, which were purchased from the China Center for Type Culture Collection (Shanghai, China), were cultured in the DMEM medium supplemented with 10% FBS (HyClone, UT, USA) and 1% penicillin–streptomycin (Beyotime Biotechnology, Jiangsu, China).

Techniques: Marker, Expressing, Western Blot, Transfection

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: GBM cell viability in the dose-dependent manner: (a) Curcumin inhibited GBM cell viability in a dose-dependent manner. Cell viability was evaluated by MTT assay for LN18 and LN428 cells treated with the indicated doses of curcumin. Values are the means ± SD from three experiments; * P < 0.05, * * P < 0.01, * * * P < 0.001; (b) MTT assays of LN18 and LN428 cells treated with 5 μM curcumin and irradiated with γ-rays and neutrons. Values are the means ± SD from three experiments; * P < 0.05, * * * P < 0.001; (c) 3D spheroid growth assay of LN18 and LN428 cells treated with curcumin and radiation for four days. Phase-contrast images indicated that untreated cells formed polarized spheroids, but cells exposed to curcumin and radiation did not. Cells exposed to γ-rays and neutron radiation (5 Gy, 5 GyE).

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques: MTT Assay, Irradiation, Growth Assay

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: The radiosensitizing effects of curcumin on GBM cells: (a) Radiosensitivity of LN18 and LN428 cell lines with and without curcumin (5 μM) after various doses of γ-ray and neutron radiation was measured by colony-forming assay. The x-axis shows the equivalent dose, expressed as GyE (Gray equivalent). Values are the means ± SD from three experiments.

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques:

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Fitting parameters α and β for survival curves in cell

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques:

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Radiation dose required for 50% cell death and radiosensitivity enhancement factor (REF)

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques:

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Effects of curcumin and radiation on apoptosis and autophagic cell death in GBM cells: (a) LN18 and LN428 cells were treated with neutron and/or curcumin for 24 h. Cell cycle distribution (subG1) was analyzed quantitatively by flow cytometry. Values are the means ± SD from three experiments; * P < 0.05, * * P < 0.01, * * * P < 0.001; (b) LN18 and LN428 cells were exposed to curcumin (10 μM) and/or 5 Gy γ-ray or neutron radiation for 48 h for Annexin V staining. Values represent means of three experiments ± SD; * P < 0.05, * * P < 0.01, * * * P < 0.001; (c) Analysis of cell death in two GBM cell lines 72 h after treatment with curcumin plus radiation using a cell death detection kit. Values are the means ± SD from three experiments; * P < 0.05, * * * P < 0.001. (D, E) TUNEL staining and cyto-ID staining of LN18 and LN428 cells with and without neutron beam or with and without curcumin treatment.

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques: Flow Cytometry, Staining, TUNEL Assay

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Detection of apoptotic cells by cell cycle detection on LN18 and LN428

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques: Control

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Detection of apoptotic cells by annexin V/PI staining on LN18 and LN428

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques: Staining, Control

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Detection of apoptotic cells by cell death kit on LN18 and LN428

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques: Control

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Effects of curcumin and neutron on ROS in GBM cells: (a) Cell lysates (30 μg) were immunoblotted (IB) with indicated antibodies; (b, c) Analysis of ROS generation in LN18 and LN428 cell line 24 h after treatment with curcumin, neutron or combination by a caspase 3 assay kit and ROS detection kit. Values are the means ± SD from three experiments; * P < 0.05, * * P < 0.01, * * * P < 0.001.

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques: Caspase-3 Assay

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Detection of ROS on LN18 and LN428

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques: Control